That Other Number - The Meaning Of Numerical Aperture In.
The magnification power of low power (lp) is 100x. The optical lens is 10 and the objective lens is 10; when the two are multiplyed together the total is 100x.
Do not use an objective with an NA of more than 1.10 Do not use an objective with less than 10x magnification Fortunately, if you plan to use a high power lens with a maximum NA of 1.25 or higher, you could just add a funnel stop in the back focal plane of the objective to lower its maximum NA to something around 1.10. You could also use a.
When imaging (see Step 3), use a high-NA objective. To generate mosaic embryos. iii. Inject 4-cell embryos with an mRNA encoding a fusion protein of interest. Alternatively, mosaic expression can also be achieved by injection of plasmid DNA (Vize et al. 1991). Because plasmid DNA is not transcribed until after the mid-blastula transition, this approach avoids early expression of the marker.
Wide FOV and high precision. FOV 6.7 times wider than typical CLSM is possible, thanks to HYBRID's wide FOV and the use of special high-NA low magnification objective lens.
Each objective housing is engraved with key specifications including the magnification, the numerical aperture, and an infinity symbol noting that it is infinity corrected. The housings have external M26 x 0.706 threads, which can be integrated into an SM1 lens tube system with the use of an SM1A27 adapter. All of the objectives found on this page have a parfocal length of 95 mm (see the Specs.
I will cover both resolution and NA in other articles, but in brief, the NA of an objective relates to the light gathering abilities whereas resolution relates to the ability distinguish details in a specimen. In this article, we will have a look at microscopy immersion techniques for imaging specimens at high magnifications whilst increasing NA and overcoming some of the limits of resolution.
Using the lowest magnification means that the specimen is far enough away from the lens in comparison to the higher magnification lenses, offering the widest field of visible range. By starting with the lowest magnification, the specimen is easier to locate, center, and focus in on. Once the specimen is in view under this magnification, it is also easier to focus on the object using the fine.